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英文 高中

請問456~

mmon in our daily lives, and toccurs when someone says mean or many forms, one of which is an active assaultL For example, in by calling him or her an widiot. "Astudent may be mocked ly least expect is that verbal abuse 4 Verbal abuse is quite col rude things to us. Verbal abuse can 1 aworkplace a supervisor may insult a(n) 2一 by his or her peers _3_ being different' What we would probabl should be 4 achid who is regularly scolded by his or her teachers or parents. There is atso 5 verbal abuse, and this occurs when aperson or a person's opinion is intentionally ignored Recent scientific research has proven that verbal abuse is damaging both emotionally and physically 6_by anxiety or depression Victims of verbal abuse usually develop low self-esteem and may pe _6_ mind are sometimes so deep that they cause post-traumatic stress _7_,theemotional scars left in their disorder which may even lead to suicide. Whats worse, some victims wind up _8_abusers themselves, hats more, brain scans have shown mainly because this is the only means of expression they Know. W 如at_ the brains of some victims look much like _9_ of disabled people, which_may explain Why verbal-apuse victims often experience memory problems and their language ability is often 2全 Hence, 代is very important that we choose and use our words wisely. (1) ( OI(A) take (B) acquire (C) classify (D) define (2)( ,) (A) competitor (B) adolescent (C) subject (D) subordinate 人 (0 (A) in (B) for (C) beyond (D) after (4)當2) (A) happened to (B) imposed on (C) based on (D) kept down (5) (2) (A) acceptable (B) consequent (C) passive (D) mutual (6) GS) 人) incensed (B) suffered (C) plagued (D) resisted (7 (人 (A) Although (B) After al (C) In addition to (D) Moreover (8) (人AA) Becoming (B) to become (C) and become (D) as becoming ( 玉 (2 (A) what (B) others (C) those (D) which 、 IN Withidrawn (D) rejected

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生物 高中

請問quantitative PCR(定量PCR)和RT-PCR是不是不相同呢? 因為在網上搜尋quantitative PCR(定量PCR)都是出現RT-PCR 另外,是否可以解釋quantitative PCR(定量PCR)呢? 因為目前在網路上看到的資訊都不太能理解

quai ve Total RNA was isolated using TBlzol reagent (Invitrogen ! fe Technologies, Carlsbad, CA, USA) according to the mar facturer's instructions. The purity and concentration ofthe BRNAw /e Jme spectrophotometer (Maestrogen, Las Vegas, NV, USA), and cDNAs Were synthesized using the miScript II RT kit (Qiagen, Germany) according to the manufacturer's instructions. evaluate the expression of MMP-1, quantitative PCR was performed using the following primers: forward, 5-TCT GIAGGTTGATCCCAGAGAGCAG-3' and reverse, 5-CAGGG TGACACCAGTGACTGCAC-3“ using EvaGreen dye (Solis BioDy Line-Gene K software (Bioer Technology Co,, Ltd., Hangzhou, China). The Ct value for each gene was normalized to B-actin using the following primers: forward, 5“-GGATTCCTATGTGG 3 and reverse, 5/- GGCTCGGGTGAGGAITCTTCATG-3/ ive expression levels Of each gene were calculated using the 2-AAct method, as previously described- RNA萃取&定量PCR 根據製造商的說明,使用 分離總RNA。使用Ma ) Maestrog 對RNA的純度和渡度進行了評價,根據製造商的說明。使用iS (Qiagen) 合成了cDNAs。為了評估MMP-1的表達。使 用以下引物(子)進行了定量PCR : 正向5-TCTGACGTTGATCCCAGAGAGCAG-3'和 向5-CAGGGTGACACCAGTGACTGCAC-3', 使用EvaGreen染料 ss BioDyne) 和Line-GeneK軟件。使用以下引物將每個基因的@ B-actin 向$:-GGATTCCTATGTGGGCGACGA-3', CGCTCGGTGAGGATCTTCATG-3'。 如前所述,使用2-se'法 LuyittogenT 生 人 oo

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