A circular 3518 bp plasmid DNA has EcoRI restriction sites at position 86 and
position 1108 and 2950; and a BamHI site at position 2623.
7.
1435; Pstl sites at
Which one of the following choices is not correct? (109**)
EcoRI digestion yields two bands in agarose gel.
B. When digestion with EcoRI and BamHI, three bands will be observed in
agarose gel.
CA
After digestion with EcoRI, Pstſ and and BamHI, fire bands will be
observed in agarose gel.
D. When digestion with EcoRV and Pstl, only two bands will be observed in
(2
to Pst I takty
E. BamHI digestion yields only one band in agarose gel.
32
agarose gel.
3518
86 EcoRI
XPSI 1108
(1435 EGORI
X
pst I za Banilts
2980
24
BamHI
2623