年級

科目

問題的種類

生物 高中

請問quantitative PCR(定量PCR)和RT-PCR是不是不相同呢? 因為在網上搜尋quantitative PCR(定量PCR)都是出現RT-PCR 另外,是否可以解釋quantitative PCR(定量PCR)呢? 因為目前在網路上看到的資訊都不太能理解

quai ve Total RNA was isolated using TBlzol reagent (Invitrogen ! fe Technologies, Carlsbad, CA, USA) according to the mar facturer's instructions. The purity and concentration ofthe BRNAw /e Jme spectrophotometer (Maestrogen, Las Vegas, NV, USA), and cDNAs Were synthesized using the miScript II RT kit (Qiagen, Germany) according to the manufacturer's instructions. evaluate the expression of MMP-1, quantitative PCR was performed using the following primers: forward, 5-TCT GIAGGTTGATCCCAGAGAGCAG-3' and reverse, 5-CAGGG TGACACCAGTGACTGCAC-3“ using EvaGreen dye (Solis BioDy Line-Gene K software (Bioer Technology Co,, Ltd., Hangzhou, China). The Ct value for each gene was normalized to B-actin using the following primers: forward, 5“-GGATTCCTATGTGG 3 and reverse, 5/- GGCTCGGGTGAGGAITCTTCATG-3/ ive expression levels Of each gene were calculated using the 2-AAct method, as previously described- RNA萃取&定量PCR 根據製造商的說明,使用 分離總RNA。使用Ma ) Maestrog 對RNA的純度和渡度進行了評價,根據製造商的說明。使用iS (Qiagen) 合成了cDNAs。為了評估MMP-1的表達。使 用以下引物(子)進行了定量PCR : 正向5-TCTGACGTTGATCCCAGAGAGCAG-3'和 向5-CAGGGTGACACCAGTGACTGCAC-3', 使用EvaGreen染料 ss BioDyne) 和Line-GeneK軟件。使用以下引物將每個基因的@ B-actin 向$:-GGATTCCTATGTGGGCGACGA-3', CGCTCGGTGAGGATCTTCATG-3'。 如前所述,使用2-se'法 LuyittogenT 生 人 oo

已解決 回答數: 1